ReLASV® Pan-Lassa NP IgG/IgM ELISA Kit – RUO (10004)


Designation: Semi-quantitative detection of anti-LASV nucleoprotein (NP) human IgG and IgM antibodies
Clade specificity: Pan-Lassa, antibodies to lineages II, III, IV nucleoprotein (NP) antigens
Applications: ELISA microplate
Storage: 2-8°C
Shipping: Refrigerated; blue ice packs

Sizing Guide


This assay is a direct ELISA detecting Human IgG and/or IgM antibody specific for LASV NP. Diluted samples, Calibrator, Positive Control, and Negative Control are incubated in microwells coated with a mixture of recombinant LASV NP antigens. Incubation allows the anti-LASV antibody present in the samples to react with the immobilized antigen mixture. After the removal of unbound serum or plasma proteins by washing, anti-human IgG (or IgM) antibodies labeled with horseradish peroxidase (HRP) are added, forming complexes with the bound IgG (or IgM) anti-LASV antibody. Following another washing step, the bound enzyme-antibody conjugate is assayed by the addition of TMB substrate. Color develops in the wells at an intensity proportional to the concentration of IgG (or IgM) anti-LASV antibody in the sample.

Optical Density (O.D.) results are obtained by reading the absorbance at 450nm (minus 620 – 650nm reference) using an ELISA plate reader. It is recommended that the user establish a cut-off for the study population using LASV sero-negative samples. It is also recommended that LF IgG and IgM positive convalescent LF sample from the study population be included in each assay as an additional reference sample.

Click here for package insert for ReLASV Pan-Lassa NP IgGIgM ELISA Kit (RUO)

Click here for SDS for ReLASV Pan-Lassa NP IgGIgM ELISA Kit (RUO)

Additional information

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