Description
SDS-PAGE analysis of full length SARS CoV-2 N 5′ 6X-HIS, produced in E. coli, purified by affinity chromatography and formulated. Full length SARS CoV-2 N is expressed as a 50 kDa polypeptide. The extract containing the full length SARS CoV-2 N protein was subjected to nickel affinity chromatography, eluted with imidazole, and resolved on SDS-PAGE (lane 2). A small fraction of the protein was was not efficiently captured by the resin and was not rerepurified (lane 3, flow through). The final, dialyzed and formulated protein was a soluble full length SARS CoV-2 N 5′ 6X-HIS protein product >95% pure. |