This assay is a direct ELISA detecting Human IgG antibody specific for LASV NP. Diluted samples, Reference, and Negative Control are incubated in microwells coated with a mixture of recombinant LASV NP antigens. Incubation allows the anti-LASV antibody present in the samples to react with the immobilized antigen mixture. After the removal of unbound serum or plasma proteins by washing, anti-human IgG antibodies, labeled with horseradish peroxidase (HRP), are added forming complexes with the bound IgG anti-LASV antibody. Following another washing step, the bound enzyme-antibody conjugate is assayed by the addition of TMB substrate. Color develops in the wells at an intensity proportional to the concentration of IgG anti-LASV antibody in the sample. Optical Density (O.D.) results are obtained by reading the absorbance (A450nm minus A620nm) using an ELISA plate reader. It is recommended that the user establish a cut-off for the study population using LASV sero-negative samples. It is also recommended that LF IgG positive convalescent LF sample from the study population be included in each assay as an additional reference sample.
ReLASV® NP Lineage II IgG ELISA Kit – RUO (10729) – Special Order
$2,000.00 – $10,000.00
Designation: Semi-quantitative detection of anti-LASV nucleoprotein (NP) human IgG antibodies
Clade specificity: Antibodies to lineage II nucleoprotein (NP) antigen
Applications: ELISA microplate
Shipping: Refrigerated; blue ice packs
Ordering: Item 10729 is a special order product, and inventory may be limited. Contact Zalgen for current status and projected availability.